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Recommended Mass Spectrometry Core Facility
Columbia University Protein Core Facility:
http://cpmcnet.columbia.edu/dept/protein
Contact:
Dr. Mary Ann Gawinowicz
212-305-3631, mag4@columbia.edu
Recommended references:
Web:
About Mascot ; Interpreting
Electrospray Mass Spectra
Book:
Protein Sequencing and ID Using Tandem Mass Spectrometry
Kendrick White Papers
June 2012:
A gene's mRNA
level does not necessarily predict its protein level
Kendrick PowerPoints
Ethanol Precipitation Protocol and
% Recovery Data
(pdf, not a PowerPoint)
2D
Method Linearity
Screening anti-HCP
antibodies using 2DE WB
32P-labeling protein kinase standards ERK, MEK &VEGF-R
Phosphoprotein Western
Blotting
PowerPoints from Dr. Kendrick's Talks at
Am. Electrophoresis Society Meetings
2012 AES Meeting:
Deglycosylation and Antibody Screening to Identify RTKs by
2DE
2011 AES Meeting:
Identifying TK Drivers in Human Tumors
2010 AES Meeting:
32P-labeled phosphorylated protein standards
2009 AES Meeting:
Biologics and HCP antibody analysis
2008 AES Meeting:
Post-translational Modification Western Blotting -
ubitiquitin-, sumo- and acetyl groups
2007 AES Meeting:
pTyr Western
blotting and RAD purification
Miscellaneous Links
Stanford's HighWire Search Engine:
http://highwire.stanford.edu/cgi/search
ExPASy Proteomics Server:
http://www.expasy.ch/databases.html
Compute pI/MW: http://www.expasy.ch/tools/pi_tool.html
Post-translational Modifications: http://themedicalbiochemistrypage.org/protein-modifications.html