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Recommended Mass Spectrometry Core Facility
Columbia University Protein Core Facility,
  website: http://cpmcnet.columbia.edu/dept/protein
Contact:
Dr. Mary Ann Gawinowicz  212-305-3631, mag4@columbia.edu
Recommended references:
  Web: About Mascot ;  Interpreting Electrospray Mass Spectra
                                                      Book: Protein Sequencing and ID Using Tandem Mass Spectrometry

Kendrick White Papers
Sept 2011: A
gene's mRNA level does not necessarily predict its protein level

PowerPoints from Dr. Kendrick's Talks at Am. Electrophoresis Society Meetings
2010 AES Meeting: 32P-labeled phosphorylated protein standards
2009 AES Meeting: Biologics and HCP antibody analysis
2008 AES Meeting: Post-translational Modification Western Blotting - ubitiquitin-, sumo- and acetyl groups

2007 AES Meeting: pTyr Western blotting and RAD purification

Kendrick PowerPoints
Ethanol Precipitation Protocol and % Recovery Data 
(pdf, not a PowerPoint)
2D Method Linearity

Screening anti-HCP antibodies using 2DE WB
32P-labeling protein kinase standards ERK, MEK &VEGF-R
Phosphoprotein Western Blotting
 

Miscellaneous Links
Stanford's HighWire Search Engine: http://highwire.stanford.edu/cgi/search
ExPASy Proteomics Server: http://www.expasy.ch/databases.html
Compute pI/MW:  http://www.expasy.ch/tools/pi_tool.html

Post-translational Modifications: http://themedicalbiochemistrypage.org/protein-modifications.html