2D ELECTROPHORESIS WITH COOMASSIE BLUE STAINING

Coomassie blue is our most quantitative stain as shown in the adjacent plot of stain density > background versus total ug rat liver cytosol loaded. for polypeptide spot 8 of MW 76,100,
pI 8.1. See Method Validation for detailed validation results confirming the linearity and reproducibility of Coomassie blue staining of our large format 2D gels. Usually 1 ug of a purified protein gives a dark spot with CB while 0.1 ug gives a faint spot. Coomassie Blue is compatible with mass spectrometry.

See Burgess Cassler, A. et. al. Clinical Chem. 35: 2297 1989 (pdf download) for validation of our standard format gels.

PRICE 2D-ES-1: 2D ELECTROPHORESIS standard format. Our standard format gel size is perfect for immunoprecipitations, subcellular fractionations, most bacterial preparations and many proteomic applications. The maximum load is 200 ug protein in a maximum volume of 50 ul. One Coomassie blue stained, 2-D gel of dried dimensions 16 cm wide x 13 cm long, is returned for each sample along with a pH gradient plot and a method description sheet suitable for reports or publications. The example below is from an Escherichia coli sample prepared in our Urea sample buffer (left) and SDS Boiling Buffer (right) run identically on a pH 4-8 IEF gradient and 10% acrylamide slab gel. The great advantage of carrier ampholine (CA) 2D electrophoresis is compatibility with SDS buffer and  ease of sample preparation. Molecular weight standards (220,000, 94,000, 60,000, 43,000, 29,000, and 14,000) appear as bands on the right side of the gel labeled with press-on numbers. One isoelectric point marker, added to each sample as an internal standard, is marked with an arrow. Our standard IEF tube gel contains 2% pH 4-8 BDH ampholines; transparency drying (STP-3) is standard. The duplicate gel rate is used for duplicate gels with different stains and also for blots, but not for different gel conditions.  See 2D-ES-5 below for our large format option.
Price: 2D-ES-1: 1 sample, $175; 2D-ES-1A:  2-3 samples, $165 each; 2D-ES-1B:  4-8 samples, $155 each; 2D-ES-1C: 9-20 samples, $146 each; 2D-ES-1D:  21-200 samples, $140 each; 2D-duplicate: $85 each.  More than 200 samples - Inquire.

PRICE 2D-ES-5  LARGE FORMAT 2D ELECTROPHORESIS  The larger size gives better resolution for proteomics applications; heavier loads may be applied to obtain more material for mass spectrometry. The maximum load is 600 ug protein in a maximum volume of 150 ul. One Coomassie blue-stained, dried 2D gel of dimensions 20 cm wide by 22 cm long is returned for each sample along with a pH gradient plot and a method description sheet.  Our standard IEF tube gel contains 2% pH 4-8 ampholines. Molecular weight standards (220, 94, 60, 43, 29, and 14 kDa) appear as bands on the right side of the gel labeled with press-on numbers. One IEF marker, added to each sample as an internal standard, is marked with an arrow. Transparency drying (STP-5, page 13) is standard. See above for Custom pH gradients. Typical protein loads are 100 (silver staining) or 600 ug (Coomassie blue) in 25-150 ul. LF Gel Price: 2D-ES-5: 1 sample $245; 2D-ES-5A: 2-3 samples $235 each; 2D-ES-5B:  4-12 samples $225 each; 2D-ES-5C: 13-200 samples $215 each,  LF-Dup: Large Format 2D duplicate gels: $100 each.  More than 200 samples - Inquire.  

Call 800-462-3417 or email 2d@kendricklabs.com for a price quote without obligation.